Amoeba proteus, w.m.

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Carolina Teacher Tips -- The Amoebae Are Hiding!

Because they are amorphous (shapeless) and nonpigmented, Amoeba proteus can be difficult to find in the shipping container. To find them, remove the lid from the shipping container and allow the bottle to remain undisturbed for 30 minutes. The amoebae suspended in the liquid medium will settle to the bottom.

After 30 minutes, quickly pour 3/4 of the liquid from the shipping container into another vessel (use a clean vessel as the decanted liquid may contain amoebae). Using a dissecting microscope or stereomicroscope, focus on the bottom of the shipping container. The amoebae should be easily observed moving along the bottom of the container.